PBT (Previous container symbol Q) container handling method
SAMPLING METHOD
Cool the dedicated container (PBT) in advance.
Points to note
Make sure that the surface of specimen in the dedicated container is below the surface of the ice water.
Do not use ice cubes.
Collect 3.0 mL of blood sample in a plastic syringe with a 20 gauge needle, if possible (19–21 gauge needle is also acceptable).
(Do not collect blood samples directly using a dedicated container [PBT].)
Points to note
Do not use any vacuum blood sampling tube, catheter, or other sampling methods.
Also, do not use any tourniquets.Avoid drawing ≥10 mL of blood.Collect blood smoothly while avoiding any damage to the vascular walls.
Remove the needle and gently open the cap of the dedicated container (PBT) to gently transfer 2.7 mL of the blood sample into the tube.Invert the tube slowly 2 to 3 times.
Points to note
Use only the dedicated container. Do not shake the dedicated container.
Promptly place the dedicated container in a rack with crushed ice and water.
Points to note
Make sure that the surface of blood in the dedicated container is below the surface of the ice water.
Proceed to the following procedures within 2 minutes.
After allowing the tube to stand in a crushed ice-water mixture for 15 to 30 minutes, centrifuge the tube at 2000 g for 30 minutes at 2 to 4℃.
(The table below shows the rotor radius and the speed [rpm] at 2000 G.)
＊ Equation of the rotating speed of the centrifuge
G=1.118×10-5×r×n2
r：radius of the centrifuge rotor (cm)
n：number of revolutions per minute (rpm)
Points to note
Make sure to centrifuge within 1 hour under cool conditions.
Collect 0.3 mL of supernatant per testing sample from a little lower than the surface into a specimen container using a micropipette.
Do not collect the supernatant close to the pellet. (Avoid collecting all plasma.)
Make sure to freeze the specimen (stability for 1 month).
Conversion table
Make sure to centrifuge within 1 hour under cool conditions.
